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Image Search Results
Journal: Molecular Autism
Article Title: The activation of mGluR4 rescues parallel fiber synaptic transmission and LTP, motor learning and social behavior in a mouse model of Fragile X Syndrome
doi: 10.1186/s13229-023-00547-4
Figure Lengend Snippet: Enhanced SV docking of Fmr1 KO synapses and lack of an effect of isoproterenol. Isoproterenol (100 µM, 10 min) increases SV docking in WT ( a, c, d ) but not in Fmr1 KO ( e, g, h ) PF-PC synapses. Isoproterenol induces an increasing trend in the number of docked vesicles (0–10 nm) in WT ( a ) (n = 250/3 and 203/3 synapses/mice: P = 0.059) at the expense of SVs within 10–20 nm, which decreased in number (*** P < 0.001). These changes are absent in Fmr1 KO slices ( e ) (n = 238/4 and 224/4 synapses/mice, 0–10 nm: P = 0.997; 10–20 nm: P = 0.988). Note the increase in SV at 10–20 nm in Fmr1 KO control as opposed to WT control slices ( ## P < 0.01). c, d, g, h Electron microscopy of PF-PC synapses. (B,F) The effect of isoproterenol on the SV distribution: WT (0–5 nm: *** P < 0.001); Fmr1 KO (0–5 nm: P > 0.05). Fmr1 KO control vs WT control at 0–5 nm ( ## P < 0.01). ( i ), Active Zone (AZ) length of WT (n = 250) and Fmr1 KO (n = 238) synapses ( P > 0.05). The values represent the mean ± S.E.M. Scale bar in ( c, d, g, h ) 100 nm. Generalized Linear Mixed Models (GLMM) with genotype and treatment as fixed effects, and animal subject as a random effect. P-values for fixed effects and their interactions were obtained with type II Wald Chi-Square tests and post-hoc tests were adjusted with the Tukey HSD method ( a, b, e, f ) and unpaired student’s t test in ( i ). n is the number of synapses, many synapses were analyzed per slice and several slices per mice
Article Snippet: P-values for fixed effects and their interactions were obtained with
Techniques: Control, Electron Microscopy